In The Name of God,

Saleh MohagheghHazratPh.D in Immunology,

Assistant Professor of Tehran University of Medical Sciences

B.Sc., M.Sc., M.P.H., Ph.D., WAIM (Integrated Medicine) FellowShip& Post Doct. (Immunotherapy & Biotherapy)

Introduction, Over twenty-six years of research conducted by me and various colleagues has resulted in the invention of an injectable, immune-enhancing and modulating drug called G2 and G2F vaccine. The G2 ingredients originated in completely natural and harmless resources, have prepared from animal lipids with addition of some other materials which I am going to mention in details.

1. All pharmacological  studies,  including:  cytotoxicity,  examination  of  the pathological effects on nine organs in mice, mutagenicity tests, amyloidosis, tissue  culture  and  animal  studies  showed  no  adverse    A  detailed description of all reports is available.
2. Evaluation of the G2 vaccine effect on mouse cellular immune system using delayed hypersensitivity and blastogenesis assay showed that this vaccine is an immune system stimulator by stimulating T helper (Th-1) cells immune response. That stimulates and strengthens this immune response more than ten times.

3. Creating an animal model of breast cancer in mice and therapeutically studies have shown that G2 vaccine can make about 42% of  mice completely cancer-free and significantly increase the life expectancy and survival.
4. Initial studies of the use of G2 vaccine in end-stage patients with various cancers showed that they had gone from complete treatment to control or noBut almost all of these patients benefited from longer life expectancy and better quality of life.
5. One of the cancer patients with concomitant asthma, recovered from asthma following the injection of the G2
6. Three volunteer patients with chronic asthma were screened for the G2 vaccine followed by 12 injections that experienced 85% asthma
7. So, we went one step back and the studies of animal model of asthma (sensitivity) were examined in one research center in Urmia city and two centers in Mashhad city. All three studies showed that the G2 and G2F vaccines reduced serum IgE levels as well as Eos. Cell counts also elevatedfor Th-1 cells cytokines pattern,
8. Phase I clinical trials of G2 vaccine in three healthy volunteers showed no adverse
9. Phase II study of human studies of the G2 vaccine among 33 asthma and allergy patients in Tabriz city has been done by a colleague in his personal clinic. He reported that between 40% and 100% of illness improved and become healthy. A detailed report of the late Dr. NasiriKhalaji is
10. The G2 and G2F vaccines of the immersion type (injectable oil type) vaccines were
11. We presented these results to the Medical Ethics Committee of the Tehran Med. , University. After two years, the committee authorized the study of the drug among asthmatic patients.
12. Phase III Clinical trials of the G2 vaccine in more than 160 asthmatic patients showed decreased serum IgE levels and Eos cell counts. Their breath problems were significantly reduced and the clinical symptoms of asthma patients showed about 70% improvement in patient satisfaction and their physician evaluation. The written report of the analysis of the findings was reflected in the research deputy of Tehran Medical Sciences of University.
13. Phase IV clinical trials of asthma patients continued until approximately one thousand asthma patients and confirmed the result of phase III. (Full written report of the late Dr. NasiriKhalaji is )
14. Simultaneous evaluation of G2 vaccine in more than one hundred asthmatic children in Rasht These surveys were conducted in accordance with a contract with the Country Support of Charitable Association of Diabetic Patients (CSCADP) in Rasht city and the results showed that about 90% of children asthma recovered. (The letter of manager of CSCADP is available). (CSCADP is the biggest NGO with more than 3.5 million members in my country).
15. All human studies in asthma and allergies have shown that the G2 vaccine has no side effects.
16. At the same time, these studies were launched at one ofTehran Hospital of Immunology and Allergy Clinic and were offered one year of asthma and allergy immunotherapy services to asthma and allergy

Mechanisms of Effect:

17. Our basic research has shown that G2 can stimulate and proliferate immune Th-1 cells. This stimulation and increased in the number and activity of Th-1 cells can be up to Th-1 cells are key cells of the immune system. Their activation stimulates a network of other cells including Natural killer cells (NK cells) via by releasing gamma-IFN. It has been shown in texts, activated NK cells release IL-12 which is an autocrine cytokine. IL-12 causes more NK cells to proliferate, and this proliferation and activity destroy all kinds of virus-infected cells as non-specific immune response.
18. This means that the proliferation and activation of NK cells causes our body to react quickly to any virus cell that is infected and immediately destroy them. It doesn’t matter what kind of virus has entered cells of the body. Our colleagues (Khodadi et al 2016) also showed that the G2 vaccine increased NKG2DR receptors on NK cells, which play an important role in the lethal activity of these By releasing viruses from dead cells, they are killed and excreted by other immune mechanisms.

This schematic figure shows how Th-1, Th-2 cells development and balance form Th-p cells. Th-2 plays a specific role in the production of antibodies like IgE resulting hypersensitivities (Asthma and Allergies). Unfortunately in most infectious and even non-infectious diseases that are associated with Th-2 cells, this will result in severe reactions. By G2 injection this balance changes in the favor of Th-1 cells increasing up to tenfold. As a result, the number and activity of natural killer cells have increased, killing viruses infected cells and releasing viruses that are destroyed by other immune mechanisms. NK cells are ready to kill any new cells that are infected with viruses and could prevent COVID-19 like infections.

19. In other words, injecting G2 vaccine enhances immunity as a sophisticated and very powerful method in the prevention and treatment of viral diseases such as seasonal flu, influenza, corona virus COVID-19, Dengue virus, Zika virus, Nipah virus It has been used in the treatment and prevention of seasonal flu and influenza in hundreds of cases and more recently in several cases in the prevention and treatment of COVID-19. That is, while having a preventive role against viral diseases, it shortens the recovery time of infected people.
20. Immunology reference books also say that between ten days to two weeks, other cells called Cytotoxic T Lymphocytes (CTLs) are formed and equipped to kill cells infected with a particular virus like COVID-19 that the body has infected with help of NK cells produced IL-12 and gamma
21. We were also able to show that the G2 vaccine can increase the number of cells with a CD+ markers (Kia SJ et 2011). In normal people the ratio is

But in many diseases, such as cancers, viral illnesses  can

be reversed and sometimes reversed, even reducing to one-fourth (1/4). This is offset by the 12 injection with interval of two per week of G2 vaccine injection.

22. Another effective mechanism of the G2 vaccine that emerged from research that are enzymes in the body outer cells membrane called MMPs (Matrix MetalloProteinases) which have role in cancers and many diseases is well known. These extracellular metalloenzymes can break down cancer cells, thereby causing metastasis and causing new cancer in another area of the body. MMPs can also cause angiogenesis in tumors to make branch vein from the main vein to feed tumors. This phenomenonis called angiogenesis. Our research on G2 vaccine has shown that can reduce the expression and activity of MMPs by up to 50%.Gene expression and activity of MMPs are increased in many diseases like: all viral diseases, asthma and allergies, inflammatory disorders of the brain like MS and subgroups of MS, rheumatic diseases in chronic wounds and also in diabetes which can sometimes be up to 64-fold higher than normal one.That is, one of the important reasonswhy G2 vaccine could help these patients via increasing their immunity.
23. Therefore, the G2 vaccine has been used and continues to be used in more than 2500 diabetic patients as recommended by the Scientific Committee of the (CSCADP) in Rasht city to improve the safety of these patients against viral flu, influenza and microbial infection during last several The numbers of injection were performed 5 up to 10 injections with interval of 5-

7 days. This has been done according to the letter from the country’s (CSCADP) scientific committee, as well as articles published in the Azad Anditishan weekly newspaper which were published years ago and in the December 2020 which because of epidemic of influenza N1H1 in our country. Furthermore, I should also add that due to the severe COVID- 19 epidemic in Rasht city, a report came from stating CSCADP that all diabetic patients who had previously received the G2 vaccine and due to quarantine conditions were able to call them. None of them have COVID-19. This means that the G2 vaccine has been shown to prevent COVID-19 in diabetic patients. Or if they have been infected, they have not shown any symptoms. This was important news, indicating that the G2 vaccine has the potential to prevent COVID-19 infection. And it can be used as a non-specific vaccine to prevent COVID-19 in high risk population.

24. The method of using G2 vaccine to prevent of seasonal flu are injections of two to three injections per week for 10 to 20 injections in normal individuals can provide relative immunity. Injections can be continued if needed. And to help treat viral flu and influenza in the first week of treatment, a daily injection will help,and if the disease persists, two injections can be taken daily. The number of injections can easily be increased in the treatment of cancers, especially because of the weaken immune system of cancerous patients.
25. Reported Complications: Following Injection of G2 Vaccines has been used by several thousand different patients, and some complications have been reported. One case of in the Urmia city:
    1. A woman presented with asthma symptoms who stated after the sixth injection that she had pain in the injection site. Therefore, injections were One vial of Betamethasone was injected in half with one hand and half with the other. After this injection, the pain disappeared and the patient’s asthma recovered.
    2. Another case was reported from Ardabil Who was a mustard gas injured man from the Iraq war against in Iran, which was similar to the patient mentioned above. This time, complications were eliminated by injecting a betamethasone into two vaccine injection sites. And the pulmonary complications of mustard gas declined dramatically.

• And the third case: a woman presented with asthma symptoms that also had a slight swelling in the armpit and breast lymph nodes after 5-6 injections. It was also recommended that she should be given a betamethasone injection at the vaccine site or use corticosteroids

1. One case was reported from the city of A 12-year-old child had symptoms of asthma, but no details were reported. It seems it was not important matter.

• In some cases, people who have received the G2 vaccine say that they have increased sweating that we do not know the

2. Some normal people after injection of G2 give them a lethargy, which seems to be the main cause of a slight drop in blood So, it seems that another application of G2 could control of blood pressure. A physician colleague who had unmanageable blood pressure mentioned that, his blood pressure was controlled after injection of G2 vaccine. So, if people blood pressure is low, instead of infusion, use a cream or honey containing G2.
3. The latest case was reported about a month ago when a woman with symptoms of asthma and allergies used the vaccine, which had

She was also advised to inject betamethasone, and his inflammation improved. However, in any case where complications occur it is recommended to inject betamethasone into the injection site as well as discontinue the G2 vaccine.

26. In several cases it was reported that people who had difficulty falling asleep at night or had to use sleeping medications were asleep. With the G2 vaccine, this can be resolved and they can easily fall asleep at night without taking any
27. Therefore, given that over 3,500 people have used the G2 vaccine and averaged between 20 up to 40 and more in cancerous patient, injections each for treatment. Therefore, over 60,000 to 100,000 injections have been performed during last 20 years and no other side effects
28. It should be noted that the immersion type of these vaccines was also developed as G2F Vaccine for long-term release, which has been shown to be highly effective for injecting tumors mainly. Also, when a relatively high safety is required, only one injection of 0.05 CC – 0.1 CC is injected subcutaneously. In some cases, Erythema or Induration may occur, sometimes with a slight itching and Then it means that good safety is activated. And if this does not happen, one week later another injection will provide sufficient and strong immunity.
29. One colleague used G2F vaccines with five times the volume of several allergic patients because of lack of G2 vaccines several years (0.5 CC volume instead of 0.1 CC was used to cause swelling of the axillaries lymph nodes of the patients. Following the above discontinued injections and one or two injections of corticosteroid this control and recovery condition was achieved. And their allergies recovered.

30.   Contraindications for the use of G2 vaccines.

These vaccines should not be used in pregnant women. Also, in all cases where immunity to the type of Th-1 cell activity is severe. Such as Helicobacter pylori and similar diseases should not be consumed.

Finally,as mentioned by WHO, and other Ministries of Health of different countries, COVID-19 has raised control points, such as regular hand washing, quarantine to control outbreaks and postponement of public outbreaks of disease control,will happen about,50- 70% of people of around the world. If we could immunize human being with G2 vaccine the rate of death will decrease significantly and we do not hesitate spreading of the virus. Because most people immunity will be high enough to become healthy, and if an individual become infects with COVID-19, he or she will become healthy after a few days, without needs hospitalized which we need at the moment.

So, for controlling this pandemic COVID-19 problem, there are two very important points that should mention.

1. One is to develop a vaccine against COVID-19, for control and prevention, which is seems not available
2. And the other way to control of distributing of COVID-19 to boost

communities’bodies’ immunity with G2 vaccine (or any things like that) to prevent or treat the infected people that have a long history of usage without any important side effects. Therefore, it seems that the G2 vaccine can partially accomplish this goal. Because it already has an operational history in diabetic asthmatic, virus infected people, cancers and other illness prevention and treatment.

Also, the G2F vaccine, which is oil type G2 vaccine and are very potent one. When injected gradually releases G2 components. As a result, it constantly stimulates and enhances immunity. In other words, with just one or two single s.c. injections (0.05-0.1CC), the normal people and patient’s immunity increases so much that he or she can resist to most of diseases such as flu, influenza viral and possibly COVID-19.As it well known that corona virus could not do important sign of illness within the people who have strong immunity.

It should be borne in mind that the preparation of the G2 vaccine is not easy and time-consuming and should take three to six months between approval and order. But at moment there are a few thousands single doses to start the injection.

Mass production requires special facilities. The most important part is to provide Clean Rooms and ampoule filing and division machines in sterile conditions. Fortunately, all the raw materials for this vaccine can be produced domestically.Moreover, the G2 vaccine last article presented as a lecture at the International Conference on Iranian herbal and Islamic Medicine, held in September 2019, was selected as the top paper and presentation.

I am readyto join the battle against ongoing pandemics and discuss in details for mass production. Please feel freeto reach out and do not hesitate to reflect on any suggestions or comments.

Yours Faithfully MohagheghHazrati

Tel. +98-9125142743 (also Whats app), dr.mohaghegh.foundation@gmail.com

References:

 

Boskabady MS, Neamati A, MohagheghHazrati S, Khakzad MR, Moosavi SH, Gholamnezhad Z. The preventive effects of natural adjuvants, G2 and G2F on tracheal responsiveness and serum IL-4 and IFN-c (th1/th2 balance) in sensitized guinea pigs. Clinics vol.69 no.7 São Paulo July 2014. https://doi.org/10.6061/clinics/2014(07)09

Hazrati SM, Aghazadeh J, Mohtarami F, Abouzari M, Rashidi A.Neuroimmunomodulation. 2006;13(4):205-8. Epub 2007 Mar 2. Immunotherapy of prolactinoma with a T helper 1 activator adjuvant and autoantigens: a case report.

Kalavi K, Mohagheghhazrati S. Treatment of DFUs: an immunologic concern.Endocrinologys Conference, Dec 19–23, Cochin, India [Oral]. (Abstract). 5 Kalavi K, Maleki A,

KalaviMergen, Hazrati SM.   Kalavi K, Moradi A, Keshtkar A, Kalavi M, Namazi G, MohagheghHazrati S. Diabetic foot ulcers: a novel treatment strategy.Irn J Med hypothesis Ideas 2008;2:1–5..Can we prevent amputation by immunologic concern? Student conference, Oct 2009, Antwerp, Belgium [Oral]. (Abstract).

Khodadadi A, Abdoli Z, Boroujerdnia MG, Assarehzadegan MA, Ghasemi M, Hazrati SM, Gerdabi ND. Cancer BiotherRadiopharm. 2016 May;31(4):119-24. doi: 10.1089/cbr.2015.1883.The Effect of G2 Adjuvant on Gene Expression and Delivery of NKG2D Receptor on NK Cells in Peripheral Blood.Original Article

KiaSJ,Sahebjamee M., Mighani G., MohagheghHazrati S., Tohidastekrad Z., Vahedi M. Shiraz Univ Dent J 2011; Vol.11, 29-34. Supplement. The Impact of Immunotherapeutic G2 Vaccine on Treatment of Oral Lichen Planus. Document Type: Original Article

Neamati A , Boskabady MH, MohagheghHazrati S , Khakzad MR, Moosav SH. Avicenna Journal of Phytomedicine 3(4):364-70 · June 2013. The effect of natural adjuvants (G2, G2F) on lung inflammation of sensitized guinea pigs.

 

Neamati A, BoskabadyMH, Tabatabaei A, MohagheghHazrati S. Iran Red Crescent Med J. 2014 February; 16(2): e14267. DOI: 10.5812/ircmj.14267 Published online 2014 February 5. Research Article. The Effect of Natural Adjuvants on Pathological Changes in Sensitized Guinea Pig Lungs.

Mirsadraee M, MohagheghHazratiS, Khakzad MR, Ghafarzadegan K, Boskabady MH. Iranian Journal of Basic Medical Sciences www.mums.ac.ir Vol. 15, No. 5, Sep-Oct 2012, 1068-1075 Dec 24, 2011 1068 Iran J Basic Med Sci, Vol. 15, No. 5, Sep-Oct 2012 Preventive Effect of Novel Bacterial Polysaccharide and Animal Splenic Protein as Natural Adjuvants on Animal Model of Asthma.

 

Shafie S, Soltani E, Soltani M, HazratiMohaghehg S. Fish Shellfish Immunol. 2018 Nov;82:115-120. doi: 10.1016/j.fsi.2018.08.011. Epub 2018

Aug 7. Adjuvant efficacy of G2 (buffalo spleen extraction) against Yersinia septicemia in rainbow trout (Onchorhynchus mykiss).